Structure of Phytoene Desaturase Provides Insights into Herbicide Binding and Reaction Mechanisms Involved in Carotene Desaturation
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چکیده
منابع مشابه
Bleaching herbicide flurtamone interferes with phytoene desaturase.
The mode of action of the furanone herbicide flurtamone and derivatives was investigated with cress seedlings and with the unicellular cyanobacterium Anacystis. Either in the light or in the dark these compounds inhibited the formation of alpha- and beta-carotene and all of the xanthophylls in the seedlings. Instead, phytoene, a precursor of colored carotenoids, was accumulated. In illuminated ...
متن کاملIn vitro and in situ inhibition of carotenoid biosynthesis in Capsicum annuum by bleaching herbicides.
Pepper leaves treated with the herbicide J852 show an accumulation of phytoene and zeta-carotene, whereas treatment with norflurazon led to an accumulation of only phytoene. The effects of these herbicides were examined in vitro after the expression of carotenoid desaturases in Escherichia coli. Whereas norflurazon is a potent inhibitor of phytoene desaturase (PDS) (I(50) = 0.12 microM) but not...
متن کاملPhytoene desaturase from Arabidopsis.
Carotenoids are isoprenyl pigments necessary for protection of the photosynthetic apparatus in a11 green plants. One of the intermediate steps in carotenoid biosynthesis is the conversion of phytoene to {-carotene via two dehydrogenation reactions catalyzed by the enzyme PDS. We have previously cloned cDNAs for this enzyme from soybean (Glycine max; Bartley et al., 1991) and tomato (Lycopersico...
متن کاملBansformation of tobacco with a mutated cyanobacterial phytoene desaturase gene confers resistance to bleaching herbicides.
Carotenoids are constituents of the photosynthetic apparatus and essential for plant survival because of their involvement in protection of chlorophylls against photooxidation. Certain classes of herbicides are interfering with carotenoid biosynthesis leading to pigment destruction and a bleached plant phenotype. One important target site for bleaching herbicides is the enzyme phytoene desatura...
متن کاملFunctional complementation in Escherichia coli of different phytoene desaturase genes and analysis of accumulated carotenes.
Three different phytoene desaturase genes, from Rhodobacter capsulatus, Erwinia uredovora, and Synechococcus PCC 7942, have been functionally complemented with a gene construct from E. uredovora which encodes all enzymes responsible for formation of 15-cis phytoene in Escherichia coli. As indicated by the contrasting reaction products detected in the pigmented E. coli cells after co-transformat...
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ژورنال
عنوان ژورنال: Structure
سال: 2017
ISSN: 0969-2126
DOI: 10.1016/j.str.2017.06.002